Ref: CFS 1462
Information Requested: An ICBE member company is interested in any guidance on using the H class Waters UPLC system. Does the H class UPLC work equally well for HPLC (High Performance Liquid Chromatography) and UPLC (Ultra Performance Liquid Chromatography) method transfer? UPLC is an upgrade on HPLC.
The company is planning on purchasing a UPLC or H Class system and would welcome feedback from members that have experience using both the HPLC and the UPLC system. The feedback would then help the company to decide which is the best system to purchase.
Objective: To find out more information that would help the member company decide the best system to purchase; and also any assistance with sourcing used systems where possible.
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From: Canty, Brenda [GPSIE] [mailto:firstname.lastname@example.org] Subject: ICBE Call For Support – Analytical UPLC Technology (H Class Waters System)
Hi Grainne, My lab has experience with using the H class UPLC for both HPLC and UPLC testing. Both techniques work well on the system. There is a certain amount of change-over required to switch between UPLC and HPLC use, similar to switching between NP-HPLC and RP-HPLC. So I would advise that you may not want to be switching usage on a weekly basis as reliability problems can arise if you’re not careful.
The system we have used is the Waters H Class UPLC. Once the instrument is properly maintained it works well.
We have found that method transfer of legacy HPLC methods may need a comparability study as there can be difference with sensitivity etc. of the detector. This would especially be the case if you plan to transfer from one instrument make to another (e.g. Agilent to Waters).
I gave Eimear your contact details
From: McCarron, Eimear [GPSIE] [mailto:emccarro@ITS.JNJ.com] Subject: RE: ICBE Call For Support – Analytical UPLC Technology (H Class Waters System)
Hi Grainne, One of our QC Analysts will assist with the information requested. Do you have a specific contact?
Eimear McCarron PhD
Quality Control Manager
Little Island, Co Cork
Phone: 021 4978305
From: Kennedy_Lorraine [mailto:Kennedy_Lorraine@Allergan.com] Subject: FW: ICBE Call For Support – Analytical UPLC Technology (H Class Waters System)
Grainne, We have used the H-Classes here in Allergan for a number of years as HPLC’s and have recently developed a UPLC method on them also. We can offer our opinions and learning’s if that is of benefit.
You can pass along my details and I can coordinate with the relevant personnel here.
TOAS Manager, Allergan Westport
Mark Croke 087-6371434 Aquilant Analytical Sciences
They sell Knauer products